VNIRTM Hoechst 33258

All Products Cell Biology  VNIR 212

Description

VNIRTM Hoechst 33258 is a cell permeable blue emitting DNA specific fluoroscent stain binding to minor groove of dsDNA with AT rich sequences. This dye can be used both on live and fixed cells. The dye is extensively used in Fluoroscence microscopy and Flow cytometry applications. The dye sometimes is also used for DNA content analysis in a sample by plotting standard curve of Fluoroscence intensity vs DNA Concentration. Hoechst 33258 is less permeable than Hoechst 33342 and doesn’t induce apoptosis.

Cat. No.
VNIR212
CAS No.
23491-45-4
Molecular weight
533.9
Molecular formula
C25H24N6O.3HCl
Appearance
Clear yellow solution
Concentration
200µM in DMSO
Spectral property
Excitation: 352nm, Emission: 461nm (DNA Bound)
Storage conditions
-20°C (protect from light)
References
Please let us know if you publish your research using VNIRTM Hoechst 33258. We can cite your reference here.
Fig: Human Keratinocytes stained with VNIRTM Hoechst 33258 (5µM)