VNIRTM HotStart master mix is an optimized ready-to-use 2X master mix for PCR amplification of any genes. To prevent non-specific amplification antibody trapped HotStart DNA polymerase is employed which needs initial activation at 95°C for 6min. This master mix is useful for efficient amplification of GC rich, low copy number genes from various template types and can be used up to 50 cycles. This master mix contains 1.5 mM MgCl2 (1X final concentration).
Cat. No.: VNIR103
- Extensively tested for reproducibility in PCR and RT-PCR.
- Enzyme tested for amplification of genes up to 8kb and GC% >60.
- Suitable for viral genes amplification as well.